TY - CHAP A1 - Tomohisa Ogawa A2 - Tsuyoshi Shirai ED1 - Tomohisa Ogawa Y1 - 2013-05-29 PY - 2013 T1 - Experimental Molecular Archeology: Reconstruction of Ancestral Mutants and Evolutionary History of Proteins as a New Approach in Protein Engineering N2 - Given the centrality of protein to many biological process, this book makes a significant contribution to the fields of healthcare and nutrition. Its chapters consider topics such as protein-protein and protein-ligand docking, and the protein engineering of enzymes involved in bioplastic metabolism. One contribution gives an overview of the In Vitro Virus (IVV) analytic method, while another shows how cutting-edge techniques in protein engineering advance our knowledge in the field of palaeontology. The book also includes a review of classic and alternative strategies when using yeasts in research, with a focus on Pichia pastoris as a host. Finally, there are two contributions on chromatography: one on the method itself, and another on its use to identify HMGB1-binding components. BT - Protein Engineering SP - Ch. 5 UR - https://doi.org/10.5772/56255 DO - 10.5772/56255 SN - PB - IntechOpen CY - Rijeka Y2 - 2019-11-17 ER -