TY - CHAP A1 - Dayong Gao A2 - Xiaoming Zhou ED1 - Igor I. Katkov Y1 - 2012-03-09 PY - 2012 T1 - Prevention of Lethal Osmotic Injury to Cells During Addition and Removal of Cryoprotective Agents: Theory and Technology N2 - Almost a decade has passed since the last textbook on the science of cryobiology, Life in the Frozen State, was published. Recently, there have been some serious tectonic shifts in cryobiology which were perhaps not seen on the surface but will have a profound effect on both the future of cryobiology and the development of new cryopreservation methods. We feel that it is time to revise the previous paradigms and dogmas, discuss the conceptually new cryobiological ideas, and introduce the recently emerged practical protocols for cryopreservation. The present books, "Current Frontiers in Cryobiology" and "Current Frontiers in Cryopreservation" will serve the purpose. This is a global effort by scientists from 27 countries from all continents and we hope it will be interesting to a wide audience. BT - Current Frontiers in Cryobiology SP - Ch. 4 UR - https://doi.org/10.5772/34473 DO - 10.5772/34473 SN - PB - IntechOpen CY - Rijeka Y2 - 2020-08-06 ER -