TY - CHAP A1 - Chiharu Sogawa A2 - Norio Sogawa A3 - Shigeo Kitayama ED1 - Hesam Dehghani Y1 - 2012-03-09 PY - 2012 T1 - Immunocytochemical Approaches to the Identification of Membrane Topology of the Na+/Cl--Dependent Neurotransmitter Transporters N2 - Immunocytochemistry is classically defined as a procedure to detect antigens in cellular contexts using antibodies. However, over the years many aspects of this procedure have evolved within a plethora of experimental setups. There are different ways to prepare a given specimen, different kinds of antibodies to apply, different techniques for imaging, and different methods of analyzing the data. In this book, various ways of performing each individual step of immunocytochemistry in different cellular contexts are exemplified and discussed. Applications of Immunocytochemistry offers technical and background information on different steps of immunocytochemistry and presents the application of this technique and its adaptations in cell lines, neural tissue, pancreatic tissue, sputum cells, sperm cells, preimplantation embryo, arabidopsis, fish gonads, and Leishmania. BT - Applications of Immunocytochemistry SP - Ch. 6 UR - https://doi.org/10.5772/34514 DO - 10.5772/34514 SN - PB - IntechOpen CY - Rijeka Y2 - 2021-06-15 ER -