TY - CHAP A1 - Amit Jathoul A2 - Erica Law A3 - Olga Gandelman A4 - Martin Pule A5 - Laurence Tisi A6 - Jim Murray ED1 - David Lapota Y1 - 2012-02-01 PY - 2012 T1 - Development of a pH-Tolerant Thermostable Photinus pyralis Luciferase for Brighter In Vivo Imaging N2 - We now find ourselves utilizing luciferase - luciferin proteins, ATP, genes and the whole complex of these interactions to observe and follow the progress or inhibition of tumors in animal models by measuring bioluminescence intensity, spatially and temporally using highly sophisticated camera systems. This book describes applications in preclinical oncology research by bioluminescence imaging (BLI) with a variety of applications. Chapters describe current methodologies for rapid detection of contaminants using the Milliflex system, and the use of bioluminescence resonance energy transfer (BRET) technology for monitoring physical interactions between proteins in living cells. Others are using bioluminescent proteins for high sensitive optical reporters imaging in living animals, developing pH-tolerant luciferase for brighter in vivo imaging, and oscillation characteristics in bacterial bioluminescence. The book also contains descriptions of the long-term seasonal characteristics of oceanic bioluminescence and the responsible planktonic species producing bioluminescence. Such studies are few and rare. BT - Bioluminescence SP - Ch. 6 UR - https://doi.org/10.5772/37170 DO - 10.5772/37170 SN - PB - IntechOpen CY - Rijeka Y2 - 2019-11-21 ER -